Comparison of Androstenedione-3-Oxime-BSA Antibody with Androstenedione-6-Carboxymethylthioether-BSA Antibody for Radioimmunoassay of Androstenedione
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چکیده
منابع مشابه
A specific radioimmunoassay for androstenedione with reduced bridge-binding.
Antibody used in a steroid radioimmunoassay raised against a steroid hapten-carrier protein conjugate may recognize both the hapten and the chemical bridge to the protein. Use of the same bridge in the radio-isotopic label may lead to higher affinity binding to the label than to the native steroid. Inhibition curves under these conditions are shallow and generally not acceptable for radioimmuno...
متن کاملInhibition of thecal androstenedione
Implants ofprogesterone on the day ofdioestrus II in the hamster induced on the following day an increase in circulating levels of progesterone (6\m=.\0\m=+-\0\m=.\7ng/ml, N = 8; sesame oil controls, <0.5 ng/ml, N = 6) and a decline in serum levels of LH (5\m=.\3\m=+-\0\m=.\4ng/ml; controls 12 \m=+-\2 ng/ml) and oestradiol (10 \m=+-\2 pg/ml ; controls 69 \m=+-\5 pg/ml). The production of andros...
متن کاملMeasurement of androstenedione levels by an in-house radioimmunoassay.
An in-house radioimmunoassay (RIA) for the measurement of androstenedione levels in serum was established and validated. Levels of androstenedione were measured by RIA using serum samples from various normal population groups and patients with congenital adrenal hyperplasia (CAH). Analytical recovery and linearity results were > 95%, while intra- and inter-assay CVs were < 10% and < 22% respect...
متن کاملInfluence of Androstenedione on Sedentary Female Rats
The pUfPose of this study was to examine the influence of androstenedione supplementation on plasma testosterone, cortisol, and cholesterol, mean arterial pressure (MAP), body weight, organ weights, and bone mineral density (BMD) in female rats. At age 10 weeks, an initial BMD measure was made. Rats in the andro group (n = 13) received implants designed to deliver 1 J.lg androstenedione per gra...
متن کاملTHE IN VITRO METABOLISM OF Al - ANDROSTENEDIONE
It has been demonstrated in this laboratory (1) that A4-androstenedione3,17, c&testosterone, and small amount of other unidentified steroids are formed when testosterone is incubated with liver slices. In order to elucidate further the course of the metabolism of these steroids, 2000 mg. of A4-androstenedione-3, 17, the chief metabolite of testosterone (l), has been incubated in lots of 100 mg....
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ژورنال
عنوان ژورنال: Folia Endocrinologica Japonica
سال: 1975
ISSN: 0029-0661
DOI: 10.1507/endocrine1927.51.7_609